- Certification: SGS
- Function: Bio-Marker
- Color: White
- Synonymy: MPEI
- M.F: C17H19IN2
- Application: Control Treatment
- Place of Origin: Shenzhen of China
- Suppliability: Large Sales
- Transport Package: 100mg, 1kg, 25kgs / Drum
- Origin: Shenzhen of China
- Environmental Protection: Yes
- Appearance: Powder
- Name: 1-Methyl-3-[(E)-2-(1-Methylpyridin-1-Ium-4-Yl)Ethe
- CAS No: CAS No:76384-55-9
- Purity: 98%Min
- Documents: SGS, MSDS
- Samples: Available
- Trademark: Dieckmann
- Specification: 100mg, 1kg, 25kgs / drum
Name: 1-methyl-3-[(E)-2-(1-methylpyridin-1-ium-4-yl)ethenyl]indole iodide
MPEI Green-Fluorescent cyanine dye (Product No.) is a cell-permeant nucleic acid stain that can selectively stain rRNA in the nucleoli. Although virtually nonfluorescent in the absence of nucleic acids, MPEI exhibits bright green fluorescence when bound to rRNA (absorption/emission maxima ~458/540 nm), but only weak fluorescence when bound to ct-DNA (Figure 1). Eukaryotic cells stained with the MPEI dye show a staining pattern consistent with that of an RNA-selective probe. Maximal fluorescence is observed in the nucleoli, with faint fluorescence found throughout the cytoplasm.
The RNA localization of the MPEI dye is further supported by RNase and DNase treatments:
a) Control treatment;
b) upon treatment with DNase, there is no significant loss of fluorescence;
c) upon treatment with RNase, the nucleolar and cytoplasmic intensities are significantly reduced, as compared with control cells; Because the MPEI dye is cell permeant, it is suitable for counter staining use in live cells.
For more detail information, please refer to (ACS Chem. Biol. 2015, 10, 1171−1175) http://pubs.acs.org/doi/abs/10.1021/cb500927r
Figure 1. Absorption and emission spectrum of MPEI without or with calf thymus DNA (ct-DNA) or 16S and 32S rRNA in T.E. buffer at pH 7.5.
Figure 2. The fluorometric titration of MPEI with the addition of rRNA in T.E. buffer. The RNA localization of the MPEI™ dye is further supported by RNase and DNase treatments
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